动物造模 Z-bio 2023-03-21 295

　　Objective To investigate the effects of arginine vasopressin 1b receptor gene knockout on plasma corticosterone content, anxiety, and social behavior in female rats.

　　Methods Using arginine vasopressin 1b receptor knockout and wild-type SD female rats as research subjects, the expression level of arginine vasopressin 1b receptor in the paraventricular and supraoptic nuclei of WT and AvpR1bKO rats was detected by in situ hybridization; The plasma corticosterone content of WT and AvpR1bKO rats at rest was analyzed by high performance liquid chromatography tandem high resolution mass spectrometry; The anxiety level of rats was evaluated by the time and frequency of WT and AvpR1bKO rats entering the central area in the open field experiment, and the time and frequency of WT and AvpR1bKO rats entering the open arm in the elevated cross maze experiment; The social ability of WT and AvpR1bKO rats was evaluated by exploring the time of unfamiliar rats in a three box experiment; The social novelty of WT and AvpR1bKO rats was evaluated based on the time spent exploring unfamiliar rats with different levels of familiarity.

　　Results There was no expression of arginine vasopressin 1b receptor in the paraventricular and supraoptic nuclei of AvpR1bKO rats; At rest, there was no difference in the plasma corticosterone content of AvpR1bKO rats compared to WT rats (P>0. 05) 05); In the open field experiment, there was no difference in the time and frequency of AvpR1bKO rats entering the central region compared to WT rats (P>0. 05) 05); In the elevated experiment, there was no difference in the time and frequency of AvpR1bKO rats entering the open arm region compared to WT rats (P>0. 05) 05); In the three box experiment, there was no significant difference in the social ability index between AvpR1bKO rats and WT rats (P>0. 05) 05); At the same time, there was no significant difference in the social novelty index between AvpR1bKO rats and WT rats (P>0. 05) 05)。

　　Conclusion Arginine vasopressin 1b receptor knockout does not affect plasma corticosterone content, anxiety level, social ability, and social novelty in female rats at rest.