动物造模,神经元损伤 Z-bio 2023-03-21 310

　　Objective To explore the effects of different exercise modes on skeletal muscle growth factors MSTN and IGF1 in rats, and to provide theoretical basis for selecting appropriate exercise modes to improve skeletal muscle function.

　　Methods Forty SD rats were randomly divided into 4 groups with 10 in each group, which were divided into a quiet control group, a continuous swimming exercise group, a high intensity intermittent swimming exercise group, and a weight bearing ladder climbing exercise group. After 8 weeks of exercise training, each exercise group was anesthetized and executed together with the quiet control group, and samples were collected. The body weight and gastrocnemius muscle mass of rats in each group were recorded, and the changes in cross-sectional area of gastrocnemius muscle were observed by HE staining. The serum levels of MSTN, IGF1, and insulin were measured by ELISA. The expression of MSTN, IGF1, and p70S6K in gastrocnemius muscle was detected by Western blot.

　　Results The weight of rats in the three exercise groups was significantly lower than that in the quiet control group (P<0.05) The gastrocnemius muscle mass in the continuous swimming exercise group was significantly lower than that in the quiet control group (P<0. there="" was="" no="" significant="" change="" in="" gastrocnemius="" muscle="" mass="" the="" other="" two="" groups="" p="">0. 05) 05); The cross-sectional area of gastrocnemius muscle in the three exercise groups was significantly lower than that in the quiet control group (P<0. 05) The cross sectional area of gastrocnemius muscle in the ladder climbing exercise group and the high intensity intermittent swimming exercise group was significantly higher than that in the continuous swimming exercise group (P<0.05) 05)。 ELISA results showed that the serum IGF1 levels in the three exercise groups were significantly lower than those in the quiet control group (P<0.05) 01), but there were no significant changes in MSTN and insulin levels. The expression of MSTN protein in gastrocnemius muscle in ladder climbing exercise group was significantly lower than that in quiet control group (P<0.05) The protein expression of IGF1 and P70S6K was significantly higher than that of the quiet control group (P<0.01) The protein expression of p70S6K in gastrocnemius muscle in the continuous swimming exercise group was also significantly higher than that in the quiet control group (P<0.01) 05)。

　　Conclusion Compared with the three types of exercise, ladder climbing exercise and high intensity intermittent swimming exercise both upregulate the gastrocnemius muscle mass index, but have different effects on MSTN and IGF1 in skeletal muscle. Ladder climbing exercise downregulated the expression of MSTN and upregulated the expression of IGF1 and p70S6K, while high intensity intermittent swimming exercise had no significant impact on the expression of MSTN, IGF1 and p70S6K in skeletal muscle. Ladder climbing exercise may increase the cross-sectional area of the gastrocnemius muscle by reducing MSTN and increasing the protein expression of IGF1 and P70S6K.