动物造模,卵巢早衰 Z-bio 2023-03-21 353

　　Objective To observe the intervention effect of resveratrol on premature ovarian failure in mice and elucidate its possible mechanism.

　　Methods Forty two-month-old female mice were randomly divided into four groups: control group, model group, 20 mg/kg RES group, and 40 mg/kg RES group, with 10 mice in each group. Measurement of mouse body weight, ovarian weight, and ovarian/mouse relative weight ratio; HE staining was used to observe the changes in the number of follicles in mouse ovarian tissue; Detection of CyclinD1, Wnt1, and β- Catenin mRNA expression; Observation of ovarian tissue by immunohistochemical staining β- Caterin protein expression; Western blot was used to detect the expression of Mvh, Oct4, SOD2, Nrf2, Bax, and Bcl-2 proteins in ovarian tissue.

　　Results The body weight, ovarian weight, and ovarian/mouse weight ratio of mice in the 20 mg/kg RES group and 40 mg/kg RES group were higher than those in the model group; Compared with the model group, the number of primordial follicles in the 20 mg/kg RES group and the 40 mg/kg RES group increased, while the number of atretic follicles decreased (P<0.05) 05), while cyclinD1, Wnt1, and β- The expression of Catenin mRNA increased (P<0.05) 05); Compared with the model group, in the ovarian tissue of mice in the 20 mg/kg RES group and the 40 mg/kg RES group β- The expression of Caterin positive protein increased in a concentration dependent manner; Compared with the model group, the expressions of Mvh, Oct4, SOD 2, Nrf2, and Bcl-2 proteins in the ovarian tissues of mice in the 20 mg/kg RES group and the 40 mg/kg RES group increased, while the expression of Bax decreased (P<0. 05) 01)。

　　Conclusion Resveratrol has a protective effect on premature ovarian failure mice, and its mechanism may be related to inhibiting ovarian cell apoptosis and activating Wnt/ β- Catenin pathway promotes the recovery of ovarian function.