动物造模 Z-bio 2023-03-21 288

　　Objective To observe the effects of long-term external or systemic administration of glucocorticoid (GC) on metabolism and organs in normal mice.

　　Methods Sixty male KM mice of clean grade, weighing 32 to 35 g, were randomly divided into 4 groups: normal control group (1 g/d of urea ointment for external use), 0 The 05% halomethasone topical group (1 g/d of halomethasone cream per animal), the prednisone intragastric group (6 mg/(kg · d) of prednisone per animal), and the dexamethasone subcutaneous injection group (0 9 mg / (kg·d))。 After continuous administration for 6 months, the effects on metabolic levels such as body weight, blood sugar, and mean arterial pressure in mice, as well as the pathological changes in the heart, liver, and other organs were observed.

　　Results The metabolic level of mice treated with long-term systemic GC significantly changed, mainly manifested by accelerated weight gain, elevated mean arterial pressure, abnormal fasting blood glucose, and oral glucose tolerance, with the most significant changes in mice treated with prednisone by gavage. In terms of organ damage, systematic application of GC to mice has significant organ damage, which can cause severe fat infiltration, inflammatory reactions, and lobular structure damage in liver tissue, with the most severe organ damage in the dexamethasone subcutaneous injection group. Long-term external use: 0 The 05% halometasone cream group and the control group had no significant effects on body weight, elevated mean arterial pressure, fasting blood glucose, and oral glucose tolerance in mice, and had no significant damage to mouse organs.

　　Conclusion Long-term external application of halomethasone cream has less side effects on the metabolic level and organ damage in mice than systematic application of prednisone and dexamethasone, with high safety. This provides clues and evidence for further guiding clinical rational use of GC in treating patients.