动物实验,莫诺苷,脑缺血 z-bo 2020-08-24 714

　　OBJECTIVE: To study the effect of moloniside on the expression of hepatocyte growth factor (HGF) and von Willebrand factor (vWF) in rat cortex of cerebral ischemia-reperfusion for 7 days.

　　Method: 25 healthy adult male Sprague-Dawley (SD) rats were prepared with a modified Zealonga suture method, and a rat middle cerebral artery occlusion (MCAO) model was prepared. Random sham operation group and model group, Missouri. There were 5 rats each in the low, medium and high dose groups. Three hours after modeling, morpholine compounds were administered intragastrically at a dose of 30,90,270 mg/kg once a day. Seven days after cerebral ischemia and reperfusion, immunoblotting and immunofluorescence were used to detect the effect of moloneside on the expression of HGF and vWF in rat cortex.

　　Results: After 7 days of cerebral ischemia and reperfusion, Western blotting significantly increased the expression of HGF protein in the model group compared with the sham operation group (P\u003c0.01). Moroniside administration group (30mg/kg, 90mg/kg, 270mg/kg) HGF protein expression level increased significantly (P\u003c0.05, P\u003c0.01, P\u003c0.001). Compared with the sham operation group, the immunofluorescence of the model group showed a significant increase in the expression of vWF (P\u003c0.001); compared with the model group the expression of vWF in the high-dose group (90 mg/kg, 270 mg/kg) Increase (P\u003c0.01, P\u003c0.001).

　　Conclusion: Carnosine can up-regulate the expression of HGF and vWF in the brain and promote angiogenesis in rats with focal cerebral ischemia.