动物实验,沙利度胺,颌骨坏死 z-bo 2020-08-24 575

　　Objective: To study the effect of thalidomide on bisphosphonate-related osteonecrosis of the jaw.

　　Method: 36 rats were randomly divided into groups A, B, and C, and treated with saline, zoledronic acid, zoledronic acid + thalidomide, respectively. Three weeks after treatment, the first molar of the left upper jaw of the rat was extracted. Four to eight weeks after tooth extraction, specimens are collected to assess jaw osteonecrosis, microangiogenesis, and apoptosis.

　　Result: At the 4th and 8th weeks after tooth extraction, no dead bones were exposed in the maxillary tooth extraction wound of the rat, but small fistulas were seen in some specimens of groups B and C. Histological examination showed that there was no dead bone in the specimens of group A, but small pieces of dead bone were found around the extraction sockets of groups B and C. Although the bone defect density of groups B and C were significantly reduced, the percentage of empty bone defects and the area of dead bone around the extraction socket were significantly higher than that of group A (P\u003c0.01). The microvessel density of group B and C was significantly lower than that of group A, and decreased by 25.87% and 55.27% at the 4th week (P\u003c0.01), and decreased by 45.62% and 72.84% at the 8th week. (P\u003c0.01); at the 4th week, the number of apoptotic cells increased by 54.80% and 87.89%, respectively (P\u003c0.01), while at the 8th week, the number of apoptotic cells increased by 208.08 % And 250.58% (P\u003c0). 01).

　　Conclusion: Thalidomide aggravates early osteonecrosis of the jaw caused by zoledronic acid; the effect of thalidomide and zoledronic acid on osteonecrosis of the jaw is related to the inhibition of microangiogenesis.