芍药苷,动物实验,神经元细胞 z-bo 2020-08-24 564

　　Objective: To establish a method to evaluate the effect of glucosinolates on the intracellular free Ca2 + concentration of rat dorsal root ganglion neurons.

　　Method: Obtain rat dorsal root ganglion by microdissection, obtain rat dorsal root ganglion (DRG), digest and screen by trypsin, use DF-12 and anti-mitotic medium instead of culture and purification using cellular immunofluorescence technology Obtain neurons and determine the purity of DRG neurons; use laser confocal microscopy imaging technology to observe the changes of Ca2 + fluorescence intensity and Ca2 + of cells and analyze the change rate of fluorescence intensity to study the concentration of free calcium ion in DRG cells And capsaicin receptors.

　　"Result: The purity of DRG cells isolated by the above method increases intracellular Ca 2+ by blocking the effect of the transient receptor potential channel activated by capsaicin, and capsaicin exceeds 95%. Can be suppressed. Eon glucoside has a similar effect to capsaicin and can prevent the influx of extracellular Ca2 +.

　　Conclusion: Pa drug may inhibit the significant increase of Ca2 + in DRG cells by acting on TRPV1 channels. This method can be used to evaluate the effect of drugs on the Ca2 + concentration in rat DRG cells.