动物实验,脓毒血症 z-bo 2020-08-24 532

　　Objective: To study the effect of dexmedetomidine on renal function and tight junction protein expression in septic rats.

　　Method: 30 SD rats were randomly divided into dexmedetomidine treatment group (Dex group), sepsis model group (M group) and saline control group (C group), with 10 rats in each group. Rats in the Dex group were injected intravenously with 5 mg/kg lipopolysaccharide (LPS) and pumped with 7 μg/kg Dex. After 15 minutes, the dose was adjusted to 5μg/kg·h, and the pump continued for 30 minutes. The model group was given LPS and normal saline in the same way. Group C always used the above method to give normal saline. After 24 hours, blood was collected, plasma was separated, and the content of creatinine (Scr) and urea nitrogen (BUN) was detected by a multifunctional biochemical analyzer. ELISA method detects the content of IL-1β and TNF-α. Western blot was used to detect the expression of tight junction protein ZO-1 and occludin in kidney tissue. Variety.

　　Result: Compared with the C group, the model group has more serious damage, more inflammatory cell infiltration and glomerulonephritis, while the Dex group has no obvious inflammatory cells and fewer abnormal cells. Compared with the model group, the damage is reduced. Renal function and inflammatory factor test results showed that compared with group C, the levels of plasma Scr, BUN, IL-1β and TNF-α in each group were significantly increased (P\u003c0.05), ZO-1 and occludin protein The expression level was reduced (P≥0.05); all indexes in the Dex group were significantly lower than those in the model group, ZO-1 and occludin (P≥0.05). Increased protein expression level (P\u003c0.05).

　　Conclusion: Dexmedetomidine can improve renal function in acute kidney injury caused by sepsis, inhibit inflammation, enhance tight junction proteins, and have a certain protective effect on the kidneys.