动物实验 z-bo 2020-08-24 611

　　OBJECTIVE: To observe the regeneration and repair effects of a new type of biofunctional composite material in SD rat skull standard bone defects, and to provide experimental data for basic research, osteogenesis and angiogenesis of bone biomaterials.

　　Method: 3-month-old male SD rats with standard skull defect models were randomly divided into different experimental groups, and the observation time was 6 weeks. The experiment is divided into CaPfromGEM21, CaPfromGEM21 + 10 ng combined with bone morphogenetic protein-2 (BMP-2), CaPfromGEM21 + 100 ng BMP-2, CaPfromGEM21+0.3 μg platelet-derived growth factor (PDGF)-BB, CaPfromGEM21+3 μg PDGF -BB and blank group. X-ray, Micro CT, hematoxylin-eosin staining (HE staining) and related quantitative analysis were performed on the specimens.

　　Results: X-ray images indicated that the bone defects in each group were repaired to different degrees, the fracture line gradually became blurred, and the bone defects in the PDGF-BB group were basically repaired. Micro CT showed that the bone defects in each group had different degrees of new bone ingrowth. The quantitative results of new bone volume indicated that the new bone in the PDGF-BB group was significantly more than that in the BMP-2 experimental group (P<0.05), which was statistically significant. There was no acute or chronic inflammatory cell infiltration in each group of HE stained sections, and uniformly stained new bone tissue was seen in the defect. Scattered new blood vessels were seen in and around the new bone. The percentage of new bone area indicated the percentage of new bone area in the 10 ng BMP-2 group. And the percentage of new bone mass in the 0.3 μg PDGF-BB group was significantly greater than that of the pure material group (P<0.05). The new blood vessel density quantitatively indicated that the new blood vessel density of the experimental group with four additional factors was significantly greater than the blank group and the pure material group (P <0.05), 4="" p="">0.05).

　　Conclusion: CaPfromGEM21 composite BMP-2/PDGF-BB has good biocompatibility, bone defect repair effect is better than simple material implantation, and it is expected to become one of the ideal new bone regeneration and repair alternative materials.