动物实验,前列腺癌细胞 z-bo 2020-08-25 481

　　Objective: To study the effect of solanine on the growth of prostate cancer cells Du145 transplanted in nude mice and its molecular mechanism.

　　Method: Using the highly malignant metastatic prostate cancer cell line Du145 as an animal model for in vivo experiments, nude mice were subcutaneously inoculated with G1/S cells to establish a nude mouse subcutaneous tumor model. One week later, the inoculated nude mice were randomly divided into two groups: solanine experimental group and normal saline blank control group. Every 3 days, 0.2 mL of solanine (50μg/mL) and normal saline were injected into the center of the solid tumor. The tumor was grown in nude mice, and the nude mice were sacrificed by cervical dislocation after 3 weeks, the tumor tissue was excised, the tumor weight was measured, and the tumor inhibition rate was calculated based on the tumor weight. Real-time fluorescence quantitative PCR and Western blotting are used to detect the mRNA and protein expression of cell cycle-related genes in nude mice. Tunel detects tumor tissue apoptosis in situ in each group of nude mice.

　　Result: The tumor formation rate of nude mice in the solanine treatment group was significantly reduced, and the tumor growth rate of nude mice was significantly lower than that of the control group (P \u003c0.01). Solanine can inhibit the mRNA and protein expression of CyclinD1, CyclinE1, CDK2, CDK4 and CDK6 genes in tumor cells, and significantly increase the expression of p21 mRNA and protein. After lycopene intervention, tumor tissue apoptosis in nude mice increased significantly (P\u003c0.01).

　　Conclusion: Solanine can promote tissue cell apoptosis in nude mice and inhibit the growth of prostate cancer cell transplantation tumors by regulating the cell cycle G1/S checkpoint.