动物实验 z-bo 2020-08-25 723

　　Objective: To study the effect of long-term rapid eye movement sleep deprivation (5 days) on depression-like behavior and amygdala monoamine oxidase A level in C57BL/6J mice.

　　Method: Adult male C57BL/6J mice were randomly divided into a blank group, a control group and a REM sleep deprivation group, and a multi-platform water environment method was used to establish a rapid eye movement sleep deprivation model. After sleep deprivation, the open field test was used to test the exercise performance of each group at different times, and the forced swimming and sugar water preference test were used to test the depression-like behavior of each group. done. Then the brain was harvested to isolate the amygdala, and the expression level of monoamine oxidase A in each group was detected by Western Blot and real-time PCR.

　　Result: Within 1-3 days after sleep deprivation caused by rapid eye movements, the exercise capacity of sleep-deprived mice decreased significantly and returned to normal on the 4th day. Forced swimming test for 7 to 14 days can prolong the immobility time of mice. The priority of sugar water will also be greatly reduced. Western blot and real-time PCR showed that the expression of monoamine oxidase A in the amygdala increased significantly.

　　Conclusion: Prolonged and rapid eye movement sleep deprivation can cause depression-like behavior in mice. This phenomenon may be related to changes in the level of amygdala monoamine oxidase.