动物实验 z-bo 2020-08-25 451

　　Purpose: To improve the stability of tannin-fixed bovine jugular venous fluid, and the effect of subsequent treatment of tannin-fixation on blood vessel thermal stability, anti-calcification ability, biomechanical properties and fiber structure.

　　Method: Use rat subcutaneous implants to fix fresh bovine jugular veins through glutaraldehyde (glutaraldehyde group), tannic acid crosslinking (tannic acid group) and tannic acid crosslinking treatment (experimental group). Calcium content, heat shrinkage temperature, breaking strength and breaking elongation measurement model and histopathological examination and other indicators.

　　Results: All indicators of the study group were better than those of the glutaraldehyde group (P0.05), and the collagen and elastic fibers of the two groups maintained good integrity.

　　Conclusion: The post-treatment of tannin cross-linking can improve the stability of the preservation solution and restore the flexibility of the bovine jugular vein, while retaining the anti-calcification ability of blood vessels, tissue strength and fiber integrity.