动物实验 z-bo 2020-08-25 568

　　OBJECTIVE: On the basis of studying the subchronic oral toxicity of diflubenzuron in rats, to preliminarily explore the effects of the poison on the blood system of rats.

　　Method: 80 SPF SD rats were randomly divided into 4 groups according to their body weight, 20 in each group, half male and female. Feed the feed containing 0, 25, 500, 10000 mg/kg diflubenzuron for 3 months, observe the clinical manifestations, food intake, and body weight of rats in each group. At the end of the experiment, determine the relevant hematological indicators, Blood biochemical indexes, organ weight, organ coefficient and histopathological changes.

　　Results: During the experiment, the eating, activity and drinking of the female and male rats in each dose group were basically normal, and no obvious signs of poisoning were seen. The blood red blood cell count (RBC), hemoglobin concentration (HGB) and hematocrit (HCT) of rats in the middle and high dose groups decreased, and the average red blood cell volume (MCV) and red blood cell distribution width (RDW) of rats in the high dose group increased. And the total bilirubin (TBIL) of male rats increased, compared with the control group, the difference was statistically significant (P<0.01 or 0.05). The spleen weight and organ coefficient of rats in the high-dose group increased, and the liver weight and organ coefficient of female rats increased. Compared with the control group, the difference was statistically significant (P<0.01). The spleens of all animals in the high-dose group and some animals in the middle-dose group were significantly enlarged, with hard texture and darker color. The pathological examination showed that the red pulp sinus of the spleen was highly expanded and filled with a large number of red blood cells, and the medulla contained hemosiderin.

　　Conclusion: Diflubenzuron has obvious damage to the blood system of rats. Long-term exposure may cause spleen enlargement and chronic hemolytic anemia in the body.