动物实验,脂肪干细胞 z-bo 2020-08-25 468

　　The bones on the scaffold are cultured in a bioreactor, which keeps the bones under mechanical stress, ensuring that the bones are the same as those in the body.

　　Now, "saving face" has a new meaning. Active bones cultured with stem cells found in fat can be used to repair faces damaged by accidents or surgery. This is the first time that a scientist has cultivated a large piece of bone in the laboratory and later transplanted it into a pig to repair the chin of the pig.

　　Researchers claim that transplantation can accurately replicate the anatomical structure of facial bones, which means that facial features can be repaired. This means that people who have suffered accidents or have bone defects can use this technology to repair their faces.

　　Dr. Gordana Vunjak-Novakovic of Columbia University said: “This is a great demand, especially for people with congenital defects, trauma, and bone repair after cancer surgery. So this is a very important step for regenerative medicine. We hope to start clinical trials within a few years."

　　This study has been published in "Science Translational Medicine". Researchers transplanted bones cultured in a bioreactor into 14 Yucatan piglets.

　　For the first time, they used a CT scan to create a 3D model of a piglet’s jaw. Then they modeled a bovine bone and peeled off the cells of the bovine bone. This leaves only a scaffold on which the researchers placed stem cells extracted from pig fat. The scaffold is then placed in a bioreactor, where stem cells can grow, and bones and blood vessels can be produced on the scaffold. The bones are also affected by the load in the reactor to simulate the pressure in the human body. This allows the bones to grow in a more natural way and provides it with a certain amount of strength. The living bone can be used for transplantation in a few weeks.

　　Scientists used stem cells extracted from fat to grow bones in the laboratory. They can generate healthy mineralized matrix and strong blood vessels, which are then transplanted into pigs to repair their jaws. The pigs will be able to eat in a few hours.

　　Dr. Vunjak-Novakovic said that a few hours after the operation, the pigs can eat.

　　Over time, this laboratory bone will be replaced by new bone formed in the body.

　　Vunjak-Novakovic added: “The role of the bones we cultivated in the laboratory is to'guide' the template, which is the active bone reconstruction, not the final implant. This characteristic determines that the bone we transplant will become a patient. A part of the bone that allows it to actively adapt to changes in the body."

　　Vunjak-Novakovic and her team are working on new methods to help repair more complex parts of the head and face.

　　They established a company called epiBone, which is planning to conduct human clinical trials.

　　The scaffold for the stem cells is made of bovine bone from which the cells have been stripped.

　　Vunjak-Novakovic added: “The quality of this regenerated tissue exceeds that of tissues generated by other methods. Tissue engineering has changed the way we achieve tissue repair, drug testing, and disease modeling. In all these different fields, we can By providing a bioengineered environment (simulating the natural environment), cells are used to serve us and generate tissues.