动物实验 z-bo 2020-08-25 557

　　Objective: To explore the importance of early prevention of fibrinolytic disorders in preventing the progression of MN.

　　Methods: Create a MN rat model based on the improved boundary method, and detect the expression levels of uPA, tPA and PAI-1 in whole blood on the weekends of formal immunization 1, 2, 3, and 4. Observe the rats with light and electron microscopes Kidney tissue, observe the pathological changes of kidney tissue, use immunohistochemical method to detect the expression of nephrin and WT1 protein in renal podocytes, and conduct related analysis.

　　Result: Compared with the normal group and the intervention group, the whole blood uPA and tPA levels of the model group were significantly reduced, and the PAI-1 level was significantly increased, but the difference was statistically significant. Yes (P\u003c0.05). The foot processes of the glomerular podocytes fuse extensively and disappear, the GBM matrix seems to be "nail-like processes", and the degree of renal interstitial fibrosis is severe. The expression of WT-1 and nephrin protein was down-regulated in the distribution area of glomerular podocytes, and the change of nephrin protein expression was statistically significant (P\u003c0.01). Correlation analysis showed that rat whole blood tPA and uPA levels were positively correlated with changes in renal tissue renin protein expression, while whole blood PAI-1 levels were negatively correlated with renal tissue renin protein expression changes.