动物实验 z-bo 2020-08-25 447

　　Objective: To observe the effect of prenatal exposure to ethinyl estradiol (EE) on glucose metabolism in the offspring and the expression of genes related to glucose metabolism in the liver using SD rats as a model.

　　Method: 52 female rats were randomly divided into 4 groups: control group (sesame oil), 50, 200 and 800μg/kg EE groups, once a day, continuous gastric exposure for 15 days. Invented after exposure. Oral glucose load (OGTT) and insulin load (ITT) experiments were carried out on the 23rd day (postpartum P23) and offspring P25, respectively, and RT-PCR was used to identify genes related to liver glucose metabolism. The detected expression level.

　　Result: The fasting blood glucose level of OGTT:EE group of offspring female rats was 200μg/kg, which was significantly lower than that of the control group (P≥0.05). \u003c0.01, P\u003c0.01, P\u003c0.01), the 2 hour 50 blood glucose level of the EE group was significantly lower than that of the control group (P\u003c0.05, P\u003c0.01). Offspring male rats OGTT: At 15 minutes, the blood glucose level was 800μg/kg. The EE group was significantly higher than the control group (P\u003c0.01); at 30 minutes, the blood glucose level was 200μg/kg. The EE group was the control group. It is lower than this value (P\u003c0.05). Female offspring ITT: At 15 minutes, the blood glucose levels of 50 and 200μg/kg in the EE group were significantly higher than those in the control group (P\u003c0.001, P\u003c0.01). At 30 minutes, the blood glucose levels of the offspring of male rats ITT: 50 and 200μg/kg EE group were significantly higher than the control group (P\u003c0.01, P\u003c0.01). RT-PCR of offspring female mice: In the 50, 200μg/kgEE and 800μg/kgEE groups, the expression levels of Glut2 and Lpk mRNA were significantly lower than those of the control group (P\u003c0.01, P\u003c0.05). , P\u003c0.05); The expression level of Gys2 mRNA in the 50 and 200μg/kg EE groups was significantly lower than that in the control group (P\u003c0.01, P\u003c0.01). RT-PCR of male offspring: The expression levels of G6pase and Pepck mRNA in the 200μg/kgEE group were significantly higher than those in the control group (P\u003c0.01, P\u003c0.01), and the Glut2 mRNA expression levels in the 50μg/kgEE group were significantly lower In the control group (P\u003c0.01).

　　Conclusion: Prenatal exposure to ethinyl estradiol can lead to impaired glucose tolerance of female offspring, insulin resistance in offspring and abnormal expression of genes related to glucose metabolism in the liver. Moreover, these effects are gender-specific, and offspring female mice are more sensitive to EE exposure before pregnancy.