动物实验,帕金森病 z-bo 2020-08-25 580

　　Objective: To study the effect of 5-hydroxytryptamine (5-HT)-7 receptor on the excitability of pyramidal neurons in the medial prefrontal cortex (mPFC) of a rat model of Parkinson's disease (PD).

　　Method: Use extracellular bioelectric recording method to observe the 5-HT7 receptor agonist AS19, which is the dense part of the substantia nigra, and the 6-hydroxyl of normal rats and PD model rats. The effect of dopamine on unilateral injury on the electrical activity of pyramidal neurons in mPFC.

　　Result: Normal rat mPFC pyramidal neurons showed three forms of excitation, inhibition and invariance both in the systemic circulation and when AS19 was administered locally. The overall response is exciting, and the inhibitory effect caused by AS19 can be controlled by GABAA. The receptor antagonist microtoxin was reversed. In PD model rats, systemic administration of AS19 may cause three responses in mPFC pyramidal neurons. Although the overall response is excitatory, the cumulative dose of the drug required to produce excitement is significantly higher than that of normal rats. Microtoxin and topical application of AS19 can partially reverse the inhibitory effect without changing the discharge of mPFC pyramidal neurons in model mice.

　　Conclusion: The activity of mPFC pyramidal neurons is directly or indirectly regulated by 5-HT7 receptors. The degeneration of the melanostriatal pathway makes these neurons weaker in response to AS19.