动物实验 z-bo 2020-08-25 549

　　Objective: To study the effect of resveratrol glycoside (PD) on liver miR-214 and liver function in ApoE-/- mice.

　　Method: Feed male ApoE-/- mice with a high-fat diet to establish a mouse AS model. ApoE-/- mice were randomly divided into 4 groups (n = 10): model group (model), simvastatin group (simvastatin), PD low-dose group (PDL), PD high-dose group (PDH) and other settings and normal The control group had 10 C57BL/6J mice of the same age. After 12 weeks of continuous administration, blood was collected to detect blood glucose, blood lipids, aspartate aminotransferase (AST), alanine aminotransferase (ALT), liver T-SOD and MDA.

　　Results: Model group mice TC, TG, LDL-C, ALT, AST and liver MDA, normal control group (P \u003cu\→ c0.01) blood glucose and serum levels and miR-214 was significantly reduced (P\u003c0. 01). The liver section showed that the cells were filled with lipid droplets of various sizes, and most liver cells showed fatty degeneration. Compared with the model group, the high-dose PD group can significantly reduce the fasting blood glucose, serum TC, TG, LDL-C, AST, ALT and liver MDA (P\u003c0.05) of the mice, compared with the PD model group , The serum HDL-C, liver miR-214 and T-SOD (P\u003c0.05) of mice in the high and low dose groups were reduced;

　　Conclusion: PD can reduce blood sugar and blood lipids in AS mice and protect liver function. This mechanism may be mainly related to PD increasing liver miR-214 levels and regulating T-SOD, MDA and other oxidative stress indicators.