动物实验,微生物,抗氧化性 z-bo 2020-08-25 485

　　Objective: To study the effects of different doses of microbial antioxidants on sleep and antioxidant properties in mice.

　　Method: 60 male Kunming mice were randomly divided into 4 groups: low, medium and high dose groups 0.5g/kg body weight, 1.0g/kg body weight, 1.5g/kg body weight microbial antioxidants were administered. In this group, normal saline was used for tube feeding and the duration of the study was 30 days. After the last gavage, sodium pentobarbital was injected intraperitoneally into each group of animals, and the sleep state of the mice was observed by the righting reflex test, and after completion, the antioxidant properties of the mouse serum were tested.

　　Result: Compared with the low-dose group and the high-dose group, the microbial antioxidants in the middle-dose group can significantly prolong the sleep time of pentobarbital sodium (P\u003c0.05). Compared with the control group, low-dose group and high-dose group, the middle-dose group significantly increased the activity of GSH-Px (P \u003cu\→ u0.01c) and SOD activity (P\u003c0.05). ). Compared with the control group, the contents of MDA and 8-ISO-PGF2α in the middle-dose group were significantly reduced (P\u003c0.05).

　　Conclusion: Antioxidants derived from microorganisms can promote sleep in mice and improve the antioxidant capacity of the human body. This effect is most important when the dose is 1.0g/kg body weight.