动物实验 z-bo 2020-08-26 473

　　Objective: To observe the effects of high-fat diet on the hypothalamus, pituitary, testicular structure and sex hormone levels in adolescent male rats, and to study the intervention effect of six weeks of free exercise.

　　Method: 3 weeks after weaning, 40 male SD rats were randomly divided into normal diet control group (C group), regular diet exercise group (CE group), high-fat diet group (D group) and high-fat diet. Divided. Exercise group (DE group). The C and D groups ate quietly for 8 weeks, and the CE and ED groups received adaptive feeding for 2 weeks, followed by 6 weeks of free-wheel exercise every day for 1 hour and 5 pm. Eight weeks later, blood was collected from the rat's abdominal aorta, serum T, E2, FSH, and LH were measured, and testicular tissue homogenate was prepared to measure T, E2, hypothalamus, pituitary and testis. Prepare tissue sections and observe structural changes with an optical microscope.

　　Results: (1) Compared with group C, the detection rate of serum T and E2 in group D decreased, and the level of T in testicular tissue was significantly decreased (P\u003c0.05), but serum E2, FSH and LH increased significantly (P \u003c0 .05); vacuole lipid droplets appear in the hypothalamus of the pituitary, eosinophils and basophils, seminiferous tubules, and sperm cells. The proportion and number of stromal cells in the testis are reduced. (2) Compared with the D group, the serum T test value and testis T value of the DE group increased, but the serum E2, FSH and LH decreased significantly (P\u003c0.05), and vacuolar lipid droplets appeared in the thalamus. The number of pituitary eosinophils increased significantly, the number of basophils did not change, and the area of the seminiferous tube of the testicular tissue was larger, but the difference was not significant.

　　Conclusion: A 6-week flywheel exercise can effectively alleviate the abnormal secretion of sex hormones and the imbalance of estrogen and androgen ratios caused by the hypothalamus-pituitary-testis in rats with high-fat diet. There is no significant effect on the pathological changes of the shaft.