-M4受体阻滞剂MT3,动物实验 z-bo 2020-08-26 495

　　Objective: To investigate the inhibitory effect of highly selective M4 receptor blocker MT3 on deprivation myopia in guinea pig myopia and its underlying mechanism.

　　Method: Three-week-old guinea pigs were randomly divided into three groups: control group, bubble deprivation group, and bubble deprivation + MT3 group. Before and after the experiment, the refractive power was measured using peeling photometry, the biological parameters of the eye were measured using A-ultrasound, and the TGF- in the retina and choroid was measured using RT-PCR. Measure the relative expression of β2mRNA.

　　Results: The right eye of the guinea pig deprivation + MT3 group developed (-1.44±0.50) D relative myopia (right eye-left eye). Compared with the right eye of the control group, the vitreous cavity and axial length were longer (0.10±0.02) mm and ( 0.14±0.07) mm (P = 0.001, P\u003c0.001, P\u003c0.001), except for myopia, the vitreous cavity and axial length increased, and simple deprivation was significantly lower than this group (P\u003c0.001, P\u003c0.001, P\u003c0.001). Simple morphological deprivation can reduce the relative expression of TGF-β2mRNA in the retina and choroid (P\u003c0.001, P = 0.014); intravitreal injection of MT3 in the retina and choroid of the eye may lead to the loss of the relative expression of TGF-β2mRNA ( P\u003c0.001, P\u003c0.001).

　　Conclusion: MT3 can inhibit the formation of bubble-blocking myopia in guinea pigs, and may play a role by up-regulating the level of TGF-β2mRNA in the retina and choroid.