动物实验,实验动物 z-bo 2020-08-26 490

　　Purpose: To use 16SRNA high-throughput sequencing technology to determine the oral flora of several commonly used experimental animals (Tibet pig, beagle, macaque, New Zealand rabbit, Wistar rat). Compare and analyze with humans. The study of oral microecological animal model provides basic data.

　　Method: Use disposable cotton swabs to collect disposable buccal swabs from Tibetan miniature pigs, beagles, rhesus monkeys, New Zealand rabbits, Wistar rats and humans, extract total DNA from the samples, and label the universal primers to use Illumina for sequencing amplification The fragments of the 16SRNAV4 region were sequenced and analyzed by BIPES and QIIME to compare the diversity and structure of the flora.

　　Result: The number of oral flora between humans and five commonly used experimental animals is very different (P\u003c0.05). Although some animals have their own unique oral flora, the oral flora of monkeys is very similar to that of humans.

　　Conclusion: Among the five animal species, according to their similarity to certain types of human oral flora, the levels of Clostridium and Porphyromonas in the oral cavity of monkeys are the most similar to those of humans. This suggests that monkeys may be animals that researchers have simulated with more appropriate oral flora. From the perspective of a specific phylum, Tibetan miniature pigs may be more suitable model animals for studying diseases related to protein bacteria; Beagle dogs are more suitable model animals for studying diseases related to spirochetes.