动物实验,磷酸激酶 z-bo 2020-08-26 500

　　OBJECTIVE: To observe the emotional response of CFA-induced inflammatory pain model rats, and to explore the relationship between the anterior cingulate cortex (ACC) phosphokinase ζ (PKCzeta) and the emotional response of inflammatory pain rats.

　　Method: 24 clean male SD rats were randomly divided into blank control group and model control group. A model of chronic inflammatory pain was established by subcutaneous injection of Freund's complete adjuvant. Before modeling (basic) and 3, 7, 14, 21, 28 days after modeling; 14, 21, 28 days after modeling, the changes in body weight and pain threshold of each group of rats were dynamically observed. We observed the changes of all rats in the elevated maze, including the total moving distance, the moving distance with the open arm, the entry time with the open arm and the percentage of the staying time with the open arm. On the 14th and 29th days after modeling, observe the total travel distance of all rats in the open area, the travel distance of the middle quadrant, the number of entrances in the middle quadrant, and the time spent by all rats in the middle quadrant. On the 14th and 29th days after modeling, Western blot was used to detect the expression of PKCzeta protein in the unaffected and affected ACC regions.

　　Result: There was no significant difference in the body weight of the two groups of rats at each time point (P \u003c0.05). Before modeling, there was no significant difference in pain threshold between the two groups of rats (P\→0.05). One day after modeling, the pain threshold of the model control group was significantly reduced (P\u003c0.05), which was significantly lower than the threshold of the entire experiment. Blank control group (P\u003c0.05). Compared with the blank control group, the ratio of the open arm movement distance and the open stay time of the model control group decreased significantly after 28 days of the model (P\u003c0.05). The central quadrant movement distance and the central quadrant entered the model 29 days after the model. The number of hours was significantly reduced (P\u003c0.05). 29 days after modeling, the PKCzeta protein expression in the ACC area affected by the model control group was significantly higher than that of the blank control group (P\u003c0.05). And the migration distance of the open arm, the percentage of staying time in the open arm, the migration distance of the center quadrant and the time of entering the center quadrant were negatively correlated with the change of PKCzeta protein expression on the affected side.

　　Conclusion: CFA-induced chronic inflammatory pain rats may show abnormal emotional behavior; chronic inflammatory pain emotional behavior may be related to the high expression of PKCzeta in the ACC area.