动物实验,糖尿病 z-bo 2020-08-26 481

　　Objective: To study the changes of SOD activity and protein expression in the main organs of diabetic rats, and to observe the effect of antioxidant N-acetylcysteine (NAC) on human antioxidant status after short-term treatment (4 weeks).

　　Methods: STZ-induced diabetic rats (group D, n = 8) simultaneously took NAC 1.5g/kg (group D +, n = 8), normal control group (group C, n = 8), and gave the same amount of physiology brine. After 4 weeks, the heart, lung, liver and kidney tissues were obtained, and using the kit, plasma total SOD, total antioxidant concentration, lipid peroxidation specific marker 15-F2t-isoprostaglandin, total superoxide dismutase (Superoxide dismutase, SOD) activity, Western blot analysis of the Cu/Zn-SOD and Mn-SOD protein expression levels of SOD subtypes.

　　Results: Compared with group C, plasma 15-F2t-isoprostaglandin, total antioxidant concentration and total SOD activity in myocardial tissue of group D rats increased significantly, but total SOD activity in plasma, lung, liver and kidney tissues Significantly lower; the expression level of Cu/Zn-SOD protein in heart and lung tissues is significantly increased, while the expression level of liver and kidney tissues is significantly reduced; liver and kidney. The expression level of Mn-SOD protein in tissues is significantly reduced, and the expression in liver tissues is significantly increased, but the changes in myocardial tissue are still unclear. AC intervention can reverse these changes to varying degrees, but it will further reduce the expression of Mn-SOD in kidney tissue.

　　Conclusion: There are tissue differences in the total SOD activity, Cu/Zn-SOD and Mn-SOD protein expression levels of various tissues in diabetic rats. The antioxidant NAC can restore antioxidant levels to different degrees in different tissues of diabetic rats.