盲肠结扎穿孔（CLP）,动物实验 z-bo 2020-08-26 457

　　Objective: To observe the effects of Trichinella spiralis and its worm-derived protein on sepsis in mice with cecal ligation and perforation (CLP).

　　Method: 80 male BALB/c mice were randomly divided into sham operation group, CLP group, Trichinella larvae (ML) pre-infection group, Trichinella larvae soluble protein (SMP) treatment group and secretory secretory protein. Separation (MES) processing group. In the pre-ML infection group, 300 Trichinella larvae were infected with oral cavity 28 days before surgery, and the other groups received intraperitoneal injection of PBS or SMP (25μg/head) or MES (25μg/head) 30 minutes after surgery. Observe the postoperative condition and 72-hour survival rate of mice after 12 hours after operation. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea nitrogen (BUN) and creatinine (Cr) were detected . The levels and levels of TNF-α, IL-6, IL-1β, IL-10 and TGF-β were observed in mouse liver and kidney tissues. Results: Compared with the sham operation group, the 72-hour survival rate, serum ALT, AST, BUN, Cr and cytokine levels of TNF-α, IL-6, IL-1β, IL-10 and TGF in the CLP group were compared with the sham operation group. -β decreases. The level was significantly increased (P\u003c0.05). The tendons of the liver are randomly arranged, hepatocytes are edema, some renal vascular glomeruli contract, and renal tubular cells are edema. Compared with the CLP group, the serum ALT, AST, Cr, TNF-α and IL-1β levels in the pre-ML infection group decreased, and the IL-10 and TGF-β levels increased (P\u003c0.05) Serum ALT in the SMP treatment group , AST, Cr, TNF-α and IL-1β levels decreased, and TGF-β levels increased (P\u003c0.05). The 72-hour survival rate of the MES treatment group was significantly improved, the serum ALT, AST, BUN, Cr, TNF-α, IL-6 and IL-1β levels were significantly reduced, and the IL-10 and TGF-β levels were significantly reduced. The level increased significantly (P\u003c0.05), and the structural damage to liver and kidney tissues was significantly reduced.

　　Conclusion: Trichinella spiralis and its worm-derived protein can reduce the release of pro-inflammatory factors and enhance the release of immunomodulators in the serum of CLP-induced septic mice. Among them, MES has a more significant effect, and can reduce the structural and functional damage of the liver and kidney.