动物实验,颅脑损伤,神经干细胞 z-bo 2020-08-26 482

　　Objective: To investigate the time course of neural stem cell (NSC) proliferation and differentiation in the brainstem area (SVZ) after traumatic brain injury.

　　Method: Use random number method to divide rats into three groups. The control group did nothing. In the sham operation group, only the skin and skull were opened. The experimental group used Ferney's method to cause head injury (TBI). Using two cell markers Nestin and BrdU, neuron-specific markers, neuron-specific enolase (NSE) and glial cell markers, and GFAP for immunofluorescence dual-labeling of three sets of brain tissue samples I ran the protein antibody. Immunofluorescence staining is used to detect the proliferation and differentiation of endogenous NSC in SVZ after TBI.

　　Results: TBI, SVZNestin/NSE, Nestin/GFAP, BrdU/NSE, BrdU/GFAP labeled positive cells increased significantly on the injured side, and began to increase on the first day after injury, and reached a peak on the third day and reached the 14th day , And recover on the 14th day. Generally, the difference between the four time points in the experimental group and the corresponding time points in the experimental group and the control group is significant, and the positive cells labeled with Nestin/GFAP increase most significantly.

　　Conclusion: After TBI, injured SVZSC are recruited to induce the proliferation and differentiation of endogenous NSC in this area. This indicates that SVZ is one of the important germinal centers for NSC proliferation and differentiation.