动物实验,DNA重组 z-bo 2020-08-26 508

　　Most researchers use Cre-LoxP recombinase system technology to knock out the target gene sequence from the genome, or insert the gene into the genome. In recent years, the technology of using compounds and light to control the Cre-LoxP recombinase system has received more and more attention. In particular, if light can be used to control the Cre-LoxP recombinase system, it will target specific biological tissues and cells to induce DNA recombination at any time. However, when the prior art uses light for control, the efficiency of DNA recombination is greatly reduced. This is also a big problem that plagues researchers.

　　In this study, we successfully developed a light-activated Cre recombinase that allows team members to link temporarily inactivated DNA recombinase (Cre) to a light switch protein and use light to control the DNA recombination reaction . done. This enzyme is called "PA-Cre". PA-Cre can be irradiated with low light (100,000 times the intensity of light commonly used in optogenetics) to achieve DNA recombination with extremely high efficiency. A short time of about 30 seconds is sufficient to induce DNA recombination. In addition, researchers have successfully used PA-Cre to perform DNA recombination at selected target sites.

　　Based on this, the researchers used PA-Cre optics to control the genetic genes in the deep body of mice. Experiments have shown that using non-invasive methods to irradiate light from outside the body can efficiently induce DNA recombination even in the liver deep in the mouse. Researchers have shown that this result can control gene activity in the body through non-invasive light irradiation. This is very important for discovering the function of genes related to diseases.