动物实验,脊髓灰质炎 z-bo 2020-08-27 559

　　Objective: To explore the pathological evaluation method of the neurotoxicity test of polio vaccine in monkeys.

　　Method: Rhesus monkeys were injected into the thalamus by bilateral injection. After 21 days of observation, an autopsy was performed. The brain and spinal cord were completely removed. After trimming, they are dehydrated and embedded. The sections were stained with HE and gallium blue and observed under an optical microscope. , Score the neurological diseases, and judge the severity of the disease based on the score. The study included 240 animals in 12 batches of experiments.

　　Result: This method is used to extract materials from different parts of the left and right brains, such as sutures, midbrain, cerebellum, pons, medulla oblongata, cervical swelling, and lumbar swelling. Refer to "Score Method for Pathological Evaluation of Spinal Cord Neurotoxicity Research" to evaluate and score the lesions of bilateral brainstem, cervical spinal cord and lumbar spinal cord; determine the degree of local lesions. The incidence of certain neurological diseases in this study was 15%. The incidence of all types of vaccine damage is similar, with the highest percentage of mild damage. More serious lesions are mainly found through type III vaccines.

　　Conclusion: The brain neurotoxicity test uses the half-section scoring method to score the lesion, which objectively reflects the neuropathy. The method is simple, easy to implement, practical, and helps to develop a unified standard. It can be used as a reference material for the pathological evaluation method of in vivo polio vaccine neurotoxicity test.