动物实验,谷氨酰胺,干细胞移植 z-bo 2020-08-27 432

　　Objective: To investigate the role of glutamine combined with cord blood mesenchymal stem cell (MSC) transplantation in intestinal ischemia-reperfusion injury.

　　Method: before transplantation, resuscitate and culture cord blood mesenchymal stem cells in vitro, and observe the position of cord blood mesenchymal stem cells after CM-DiI fluorescent labeling. Eighty SD rats were randomly divided into normal control group, ischemia-reperfusion injury group, glutamine group, MSC transplantation group and combination group, 15 rats in each group. The control group received normal saline enema, the injury group received TNBS (S ethanol dilution) enema, one hour after TNBS modeling, the glutamine group received 0.45 g/kg glutamine in the tail vein, and the MSC transplantation group received 1010 in the tail vein once. /L cord blood mesenchymal stem cell suspension, the combination group was given 0.45 g/kg glutamine + cord blood mesenchymal stem cell suspension 1x1010/L in the tail vein. done. ELISA method was used to detect the levels of midgut fatty acid binding protein (IFABP), interleukin 6 (IL-6) and superoxide dismutase (SOD) in serum of rats in each group. After glutamine combined with MSC and RT combined transplantation, the expression of caspase-3, NF-kB, Bcl-2 mRNA and protein in rat intestinal mucosal epithelial cells was detected after 1 and 3 hours of perfusion-by PCR and Western blotting .

　　Result: The distribution of transplanted MSC cells between intestinal mucosal lymphoid tissue and glandular epithelial cells was observed by fluorescence tracing, and MSC may be involved in the repair process of intestinal ischemia-reperfusion injury. It has certain properties. Compare the changes of serum SOD, IFABP and IL-6 levels in rats of each group. Compared with the glutamine group, the MSC transplantation group and the combination treatment group, the serum IFABP and IL-6 levels of the injured group were significantly higher than those of the control group. SOD content was significantly lower than the control group, but the glutamine group, MSCs transplantation group and the combination group were significantly higher than the combination group. Obviously (P0.05). Compared with the control group, the mRNA and protein expressions of Caspase-3 and NF-kB in the intestinal mucosal epithelial cells of the injured group were significantly up-regulated, and the mRNA and protein expressions of Bcl-2 were significantly down-regulated. (P0.05), but compare the two groups with the combined group. The difference is significant (P\u003c0.05).

　　Conclusion: After glutamine group and MSC transplantation, the degree of intestinal ischemia-reperfusion injury in rats was significantly reduced. It may reduce intestinal mucosal ischemia and reperfusion by inhibiting the expression of caspase 3 and NF-kB and promoting the expression of Bcl-2.