动物实验 z-bo 2020-08-27 573

　　Objective: To study the effect of low-selenium diet on liver and kidney of rats, and the effect of macrophage polarization on M1 and M2 in liver and kidney fibrosis.

　　Method: Feed rats with low-selenium and normal diet to establish low-selenium animal model. According to the method of random number table, 12 females and 12 SD rats were divided into 2 groups: control group (group Z, feed selenium 0.18mg/kg), low-selenium group (group S, feed selenium 0.02mg/). Rats were sacrificed 109 days after feeding. The pathological changes of liver and kidney were observed by HE staining. The number of CCR7, CD206 and CD163 positive cells in liver and kidney tissue was determined by SP method. Detected by histochemistry.

　　Results: (1) Liver and kidney fibrosis in the low-selenium group was heavier than the control group; (2) Female and male rats, comparison of the liver CCR7 and CD206 staining intensity of the low-selenium group with the group (female, male, female, male, P) . \→ 0.05) There is no statistical difference in the comparison. The intensity of CD163 staining was lower than that of the control group (P 0.05 for women and men), while the control group of distal tubules was slightly lower than that of the control group (P\→0.05 for women, P0.05 for men, P0.05 for men). Women did not. Statistically significant, while men are statistically significant.

　　Conclusion: The liver and kidney of rats in the low-selenium group are obviously fibrotic. Low-selenium may inhibit the polarization of macrophages into M2 type.