动物实验,肾上腺素 z-bo 2020-08-27 475

　　Objective: To study the effects of different doses of monosinapine combined with isoproterenol on hemodynamics, cardiac index and right heart hypertrophy index in rats.

　　Method: 64 SD rats weighing 200-250g, half male and half male were randomly divided into an empty control group (n = 16) and a model group (n = 48). The model components are the high-dose group (n = 16): an intraperitoneal injection of 80 mg/kg of morphine; intraperitoneal injection of 10 mg/kg of isoproterenol for 1 week; the medium-dose group (n = 16): 55 mg Single crocroline/kg intraperitoneal injection; 8 mg/kg isoproterenol intraperitoneal injection for 1 week; low-dose group (n = 16): 30 mg/kg bicrotaline intraperitoneal injection. , 3 mg/kg isoproterenol was injected intraperitoneally for 1 week. After feeding the animals for 6 weeks, the pulmonary artery pressure and right ventricular pressure were measured, and the heart index and right ventricular hypertrophy index were measured.

　　Results: The low-dose average pulmonary artery pressure and right ventricular systolic pressure did not change significantly compared with the control group; the average pulmonary artery pressure and right ventricular systolic pressure of the average dose group changed significantly. (P\u003c0.05) However, there was no significant difference between the cardiac index hypertrophy and the right ventricular hypertrophy index in the low-dose group. In the medium-dose group, the difference in cardiac index was very significant (P≥0.01), and the index of right ventricular hypertrophy was also significantly different (P≥0.05).

　　Conclusion: Monocrotaline 55 mg/kg is used for one injection, indicating that 8 mg/kg of isoproterenol has been injected continuously for 8 weeks. It not only ensures the survival of rats, but also ensures the normal formation of pulmonary hypertension. Momordica charantia and isoproterenol can cause pulmonary hypertension in rats and suppress the heart.