动物实验,心肌球源性心肌干细胞 z-bo 2020-08-27 460

　　Objective: To investigate whether the ventricular cardiomyocyte extracellular matrix (ECM) can promote the differentiation of cardiomyocytes (CDC) into cardiomyocytes, vascular endothelial cells and vascular smooth muscle cells, and can improve the structure and function of the rat heart after myocardial infarction.

　　Method: Use the myocardial tissue mass culture method to cultivate the CDC, use the decellularization method to compose the ECM, ligate the anterior descending branch of Wistar rats, and establish an acute myocardial infarction model. IMDM (group I), ECM suspension (group E), IMDM solution containing 106 CDCs (group IC), ECM suspension containing 106 CDCs (group EC) were injected into the myocardial infarcted myocardium, each group has only 6 large mouse. Three weeks later, the cardiac function was evaluated by cardiac color Doppler ultrasound, the percentage of myocardial fibrosis positive area was analyzed by Masson staining, and the vWF and α-SA in CDC were analyzed by immunofluorescence. , Qualitatively analyzed the expression of α-SMA.

　　Results: Three weeks after myocardial infarction, the EC group had the highest left ventricular ejection fraction and short axis contraction rate (FS%), and the EC group had the lowest percentage of myocardial fibrosis positive area after myocardial infarction. The EC group had a high percentage of cells in CDC. Endothelial, cardiac muscle and smooth muscle showed positive differentiation, P≥0.05

　　Conclusion: ECM derived from myocardial tissue can promote the differentiation of CDC embedded in myocardial infarct tissue into myocardium, endothelial cells and smooth muscle cells. Combining CDC and ECM for transplantation can maximize the structure and function of the heart.