动物实验,子宫内膜异位 z-bo 2020-08-27 463

　　Objective: To understand the effect of hypoxic preconditioning (HPC) on the formation of ectopic lesions in a rat model of endometriosis (EM), to further clarify the molecular mechanism of the occurrence and development of EM, and to prevent and treat it. Provide new ideas.

　　Method: The Lewis female rats were randomly divided into two groups, treated with HPC (8% O2), normal pressure and normal oxygen (21% O2) for 8 hours, and then the uterine tissue was used as the abdominal wall of normal rats. Ported to. On the 14th day after transplantation, the volume of transplantation foci was recorded, and serum and ascites, as well as uterine tissue and transplantation foci before transplantation were detected by ELISA, immunohistochemistry, Western blot, RT-PCR and Tunnel methods.

　　Results: HPC significantly up-regulated rat uterine vascular endothelial growth factor (VEGF) mRNA (P\u003c0.01) and induced the expression of hypoxia inducible factor (HIF)-α (P\u003c0.01). can increase. The size of transplanted lesions formed by HPC transplanted uterine tissue increased significantly (P\u003c0.01), the apoptotic rate of tissue cells decreased (P\u003c0.01), and the expression of VEGF mRNA was up-regulated (P\u003c0.01). \u003c0.01), HIF-α (P\u003c0.01) VEGF (P\u003c0.05), CD31 (P\u003c0.01), Ki67 (P\u003c0.01) and Bcl-2 (P\u003c0 05) were significantly increased, and the serum and ascites VEGF content of HPC uterine tissue transplantation rats also significantly increased (P\u003c0.01).

　　Conclusion: HPC promotes angiogenesis in transplanted lesions by activating the HIF-1α/VEGF system and up-regulating the expression of Bcl-2 anti-apoptotic factors. It promotes transplantation by inhibiting cell apoptosis and promoting its proliferation. The lesion grows. Based on this, in high-risk subjects or patients with recurrent EM, avoiding repeated hypoxic stimulation of endometrial normal tissue and activating the HIF/VEGF system before the endometrial tissue falls off can be expected as a developmental measure to inhibit Human intervention controlled by EM will control the occurrence of EM.