动物实验 z-bo 2020-08-27 473

　　Objective: To evaluate the mutagenicity of clam meat hydrolysate and provide experimental data for the development and utilization of clams. Methods: Ames test, mouse multicolor red blood cell bone marrow micronucleus test and CHL cell in vitro chromosomal aberration test were used to detect the mutagenicity of the three mutagenesis tests.

　　Result: The colonies of Amestest Chinese clam hydrolysate in each dose group were within the normal range, and no colonies were more than twice the natural colonies. In the presence and absence of S9, the results of these five test strains were all negative. .. In the in vitro chromosomal aberration test of CHL cells, the chromosomal aberration rate of each dose group of clam dose meat hydrolysate was not significantly different from that of the negative control group (P\→0.05), and the result was negative. In the mouse bone marrow micronucleus test, the micronucleus rate of each treatment group was compared with the negative control group, and there was no significant difference (P\→0.05), and the result was negative.

　　Conclusion: Within the conditions and scope of the experiment, clam meat hydrolysate has no potential mutagenic effects.