动物实验,槲芪散,肝癌 z-bo 2020-08-27 474

　　Objective: To investigate the role of telomerase in the formation and development of liver precancerous lesions, and to explore its prescription drug mistletoe and its regulation mechanism.

　　Methods: The rats were divided into model groups, HQS high-dose group [8g /(kg·d)], low-dose group [4g /(kg·d)], mistletoe basic group [8mg /(kg·d)] . Do it. )] and normal people. The classical Solt-Farber two-step method was used to replicate the rat liver precancerous lesion model, and histochemical method was used to detect the expression of γ-glutamyl transpeptidase activity and immunofluorescence in liver tissue. Detect the expression of AFP in liver tissue. Quantitative Telomerase Detection Kit (QTDKit) is used to determine telomerase activity in liver tissue, immunohistochemistry is used to detect the expression of NF-κBP65 protein in liver tissue, and Western blotting is used to detect cytoplasm. Determine the content of IκB-α in protein.

　　Results: After HQS and mistletoe total alkali treatment, compared with the model group, the area of γ-GT-positive foci and the number of AFP-positive cells in the liver were significantly reduced (P\u003c0.05). In addition, compared with the NF-κBP65 model group, the number of positive cells in the liver of the treatment group was significantly reduced (P\u003c0.05). After treatment, the content of IκB-α protein in the cytoplasm increased compared with the model group, and the difference was statistically significant (P\u003c0.05).

　　Conclusion: HQS and mistletoe total alkaloids may inhibit the expression of telomerase activity in liver precancerous lesions. Its effect is to increase the expression of IκB-α by inhibiting the overexpression of apoptosis-related gene NF-κB, thereby reducing telomeres.