动物实验,丹酚酸A,心肌损伤 z-bo 2020-08-27 440

　　Objective: To observe the effects of salvianolic acid A (SAA) on heart rate variability (HRV) and myocardial injury in rats with myocardial ischemia-reperfusion injury (myocardial ischemia-reperfusion injury, MI/RI). Methods: 60 SD rats were randomly divided into 6 groups, namely sham operation group, MI/RI model group, low SAA, medium and high (0.5mg/kg, 1mg/kg and 2mg/kg) dose groups. Divided into a positive control group (2 mg/kg diltiazem) with 10 animals in each group. The MI/RI rat model was established by ligating the anterior descending branch of the left coronary artery in rats, and corresponding drugs were given before the ligation. Observe and analyze the changes in the J point and HRV of the ECG during this period. At the same time, after reperfusion, double staining with Evans blue (EB) and 2,3,5-chlorotriphenyltetrazolium chloride (TTC) was used to determine the myocardial infarct size and plasma aspartate aminotransferase (AST). , Lactate dehydrogenase (LDH), creatine kinase (CK) and its isoenzymes (CK-MB), superoxide dismutase (SOD), measurement of malondialdehyde (MDA) and nitric oxide (NO) content .

　　Results: Compared with the MI/RI model group, the SAA low, medium and high dose groups and the diltiazem group can significantly reduce the ∑-J value and the average J point of rats during reperfusion (P\u003c0.05, P\u003c0 .01), reduce the area of myocardial infarction and inhibit the increase of AST, CK-MB and CK activity (P\u003c0.05, P\u003c0.01); the standard deviation of the R interval (SDNN) and the adjacent RR interval will be significant By increasing the square root root mean square (RMSSD), HRV trigonometric function (tr.Ind) and the high frequency (HFnu), low frequency (LFnu) and the square sum of LF/HF, it reduces the ratio (P\u003c0.05, P\ u003c0.01), but also significantly increased the NO content and MDA content (P\u003c0.05).

　　Conclusion: Salvianolic acid may increase HRV and reduce the area of myocardial infarction. The mechanism of action is related to the antioxidant effect of salvianolic acid A.