动物实验 z-bo 2020-08-27 531

　　Objective: To study the effects of chronic restraint stress with different restraint times on depression-like behaviors in male and female SD rats, and to provide a basis for choosing the sex and duration of experimental animals for chronic restraint stress models.

　　Method: SD rats (male and female) were restricted for 10 days and 14 hours a day for 28 days to establish a chronic restraint stress model. Using experimental techniques such as weight monitoring, sugar water preference experiment, autonomous activity experiment, novelty experiment, etc., observe the depression-like behaviors of male and female SD rats caused by chronic restraint stress in different restraint periods.

　　Results: In 14h/28d CRS male and female rats, exercise time and central area activity time were shortened, glucose preference index was reduced, the latency of exploring new sugar increased, and the time and frequency of exploration had been reduced. The results of these experiments are significantly different from those of the control group. In the 10h/28d CRS group, male and female rats compared with the control group, the above experimental results only showed significant differences in each index. Compared with male and female rats, the CRS male group had a significantly lower sugar preference index at 14h/28 days, while the corresponding female rats did not. The exercise time of the central group of the 10h/28d CRS male group was less than that of the control group, which significantly prolonged the waiting time for exploring new species and shortened the exploration time, but it was significantly extended between the corresponding female rats. no difference.

　　Conclusion: 14 hours / 28 days CRS male and female mice showed obvious depression-like behaviors in the sugar water preference experiment, voluntary activity experiment and novelty experiment. However, only the 10-hour/28-day CRS male group showed a particularly depressive behavior tendency. Chronic restraint stress is more likely to cause depression-like behavior in male rats.