动物实验,急性肺水肿 z-bo 2020-08-27 537

　　OBJECTIVE: To investigate the changes of malondialdehyde (MDA), superoxide dismutase (SOD) and interleukin 6 (interleukin 6, IL-6) in lung tissue in a rat model of acute pulmonary edema caused by acute hypoxia.

　　Methods: 50 adult Wistar rats were randomly selected from group A (normal group), group B (acute pulmonary edema model, hypoxia for 24 hours), and group C (acute pulmonary edema model, hypoxia for 48 hours), group D ( Acute pulmonary edema model, hypoxia 72h) and E group (acute pulmonary edema hormone treatment group, hypoxia 72h). Acute pulmonary edema models were established by intraperitoneal injection of 6% ammonium chloride into rats in groups B, C, D and E. Group E received 6.0 mg/kg dexamethasone through the tail vein 30 minutes after injection of 6% ammonium chloride. .. 24, 48 and 72 hours after successful modeling, 4 groups of rats were killed, rat plasma was separated, rat lungs were collected, and 10% lung tissue homogenate was prepared by ELISA. IL-6 was detected in MDA, SOD and lung tissue, and IL-6 content and activity were detected in plasma.

　　Result: The three groups of B, C and D showed obvious edema and wet weight of the rat lung tissue. Compared with group A, the difference was statistically significant (P0.05). Long-term pulmonary edema will increase the MDA of the lung tissues of C and D rats, reduce SOD and increase plasma IL-6. Compared with group A, the change is significant, and the difference is statistically significant (P\u003c0.05). Compared with group D, lung tissue MDA of rats in group E decreased, SOD increased, and plasma IL-6 decreased. The difference was statistically significant (P\u003c0.05).

　　Conclusion: The occurrence of acute pulmonary edema is related to oxidative stress. Decreased body's antioxidant capacity and increased free radicals are important mechanisms for the development of pulmonary edema. The levels of IL-6, SOD and MDA in lung tissue are of great significance to disease progression.