动物实验,血核苷酸 z-bo 2020-08-27 512

　　Objective: To study the effect of benzene on the liver and kidney function and blood nucleotide concentration in Wistar rats.

　　Method: Give Wistar rats different concentrations of benzene 21d, use an automatic biochemical analyzer to detect the levels of different blood components, and use enzyme-linked immunosorbent assay (ELISA) to measure the loops in the blood. Guanine (cGMP) and heat shock protein 70 (HSP-70) levels can detect adenosine monophosphate (cAMP) and cyclic phosphate.

　　Result: When Wistar rats were fed with benzene doses of 0.19, 0.38 and 0.76g/(kg·bw) for 21 days, the total bilirubin level in the animal plasma was 134.40%, which was 173.63, compared with the control group. % And an increase of 254.75%. Aspartate aminotransferase increased by 70.76%, 85.44%, 106.61%, creatinine increased by 24.54%, 67.46%, 84.50%, creatine kinase 151.35%, 180.85%, 245.54%, urea [0.38, 0.76g / ([ (kg· bw) group] increased by 0.48%, increased by 23.43%; alanine transferase level exceeded the upper limit of detection, both of which were 1186.50%; compared with the control group, cAMP levels increased by 41.84%, 264.02%, 314.23%, and cGMP was 31.29%. 40.46. Compared with the control group, %, 272.14%, HSP-70 increased by 82.11%, 187.37%, 1484.21%, and amylase levels decreased by 62.03%, 63.66%, 64.45%.

　　Conclusion: Benzene pollution can change the ratio of cAMP to cGMP in the blood, causing metabolic disorders and leading to liver and kidney dysfunction or diseases.