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Genotype (genetic type) refers to the type of genes related to the traits contained in the DNA of an organism...
2020-10-14The Martin Evans Institute has successfully cultivated a pluripotent stem cell line from the internal cell mass of early embryos, which can grow in vitro and can differentiate into other cells even if it is reinjected into germ cysts , These cells (i...
2020-10-14Studies have shown that retroviruses, especially lentiviral vectors, can effectively introduce foreign DNA into egg cells...
2020-10-14DNA microinjection is the earliest, most mature and most widely used transgenic technology...
2020-10-14\"Point-specific recombination system\" includes Cre-LoxP system and FLP-FRT system. So far, the most widely used method is Cre/LoxP-mediated point-specific recombination system...
2020-10-14Inducible Gene Expression System (Inducible Gene Expression System) allows introduced genes to regulate gene expression levels at a specific time or on specific tissues or cell types...
2020-10-14
The blood samples of clinical leukemia patients cultured and passaged in the abdominal cavity of mice, bone marrow samples or non-solid tumor ascites samples are equal amounts of balanced salt solution (PBS, etc.)...
2020-10-14How to extract DNA correctly?...
2020-10-14Tail vein injection in rats is a common operation, but it is a bit difficult, but if there is no guidance, it may be a little confusing at first...
2020-10-14The selection of donor mice, excessive ovulation and reproduction should be based on the purpose of constructing transgenic animals to determine the type and pedigree of donor mice...
2020-10-14Stem cell research is one of the fastest growing and most valuable cutting-edge technologies in the field of life sciences in recent years. It was published in Science and Technology in 1999, 2000 and 2003 by the journal Science...
2020-10-14
CSU-23 culture medium is a phosphate buffer without NaHCO 3. The physiological pH phosphate buffer is composed of dihydrogen phosphate anions and dihydrogen phosphate anions with a molar ratio of 3:1...
2020-10-14HIV (HIV) is an effective and cunning retrovirus. When HIV introduces its DNA into the genome of the host cell, it can incubate for a longer period of time and can remain dormant for several years...
2020-10-14![[Animal experiment]-Analysis of in vitro fertilization technology in mice](/./uploads/images/20201014/7521963e8b308.jpg)
Remove the collected egg stromal cell-oval complex with 0.1% hyaluronidase to remove cumulus cells, and place the cells in TYH droplets...
2020-10-14According to the principle of randomization, 24 male Wistar rats (weight 190-250 g) were numbered according to their body weight, and a random list method was used...
2020-10-14
How to make transgenic mouse F0?...
2020-10-14