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Animal experiment experience: mouse tail vein injection

Animal experiment experience: mouse tail vein injection

The mouse must be fixed and the tail cannot be moved during the injection. I fixed it with a mouse holder, and then another person gently tightened the bottom of the tail with his fingers...

2020-08-13
What is the blood-brain barrier function of experimental mice?

What is the blood-brain barrier function of experimental mice?

The blood-brain barrier is composed of the pia mater, the capillary wall of the choroid plexus and the glial membrane formed by the glial cells outside the wall. It acts as a natural barrier and can block pathogenic microorganisms and toxic products,...

2020-08-13
How to do animal experiments for extraction and electrophoresis of total RNA?

How to do animal experiments for extraction and electrophoresis of total RNA?

RNA is a very important biological macromolecule in the process of gene expression in life activities, such as mRNA, which carries all the coding information of DNA...

2020-08-13
What is rapid and efficient gene knockout technology for stem cells?

What is rapid and efficient gene knockout technology for stem cells?

The Sloan-Kettering Institute and other researchers in the United States created the pluripotent stem cell genome editing platform iCRISPR...

2020-08-13
How to operate the paraffin section of experimental mouse liver tissue?

How to operate the paraffin section of experimental mouse liver tissue?

Formaldehyde should be used to infuse the fresh liver of experimental mice. Formaldehyde can fix general tissues, but dissolve glycogen and pigment....

2020-08-13
What is the method of dissecting experimental white mice?

What is the method of dissecting experimental white mice?

Learn how to dissect laboratory mice and understand the common features of mammals and rodents...

2020-08-13
How to prepare experimental animal models of acute pancreatitis?

How to prepare experimental animal models of acute pancreatitis?

Animal models of pancreatitis are the best way to study the etiology of pancreatitis and explore clinical treatment methods...

2020-08-13
Scientists use TALEN technology to successfully obtain miRNAs gene knockout mice

Scientists use TALEN technology to successfully obtain miRNAs gene knockout mice

Scientists have discovered that a mouse model of miRNAs gene knockout can be obtained through TALEN technology, successfully solving the problem of small genes (such as miRNAs) and gene knockout containing repetitive sequences that were difficult to ...

2020-08-13
Application of non-surgical embryo transfer technology in mouse animal experiment

Application of non-surgical embryo transfer technology in mouse animal experiment

Mice is an important model animal in the research of developmental biology and reproductive biology. Its embryo transfer technology is an important link in the research of in vitro fertilization, chimerism, transgene, cloning and embryo freezing seed...

2020-08-13
What method is used to non-invasively measure the blood pressure of experimental mice?

What method is used to non-invasively measure the blood pressure of experimental mice?

In the past 20 years, research scientists have been looking for more accurate non-invasive methods for measuring blood pressure in experimental mice. More accurate non-invasive methods to measure rodent systolic blood pressure, diastolic blood pressu...

2020-08-13
How to obtain materials and embed the trachea and eyes of TEM mice?

How to obtain materials and embed the trachea and eyes of TEM mice?

Prepare materials and solutions in advance to repair and prepare necessary equipment, and pre-cool them in a refrigerator at 4°C. The trachea and ophthalmic lenses are either quickly rinsed with normal saline or separated, and then directly placed in...

2020-08-13
How to optimize the isolation and purification method of adult porcine islets in animal experiments?

How to optimize the isolation and purification method of adult porcine islets in animal experiments?

Adult hybrid pigs, aged 12 months, weighing about 150 kg, are slaughtered from the pigs and blood is collected, the pancreas is quickly taken out under relatively sterile conditions, and stored in Hanks’ solution at 4°C In the laboratory. Sent to. Ti...

2020-08-13
Sperm abnormalities in mice are genetically controlled and have high heritability

Sperm abnormalities in mice are genetically controlled and have high heritability

Sperm abnormalities in mice are genetically controlled and have high heritability...

2020-08-13
The effect of hypothyroidism on learning and memory in young rats-【animal experiment】

The effect of hypothyroidism on learning and memory in young rats-【animal experiment】

Objective To explore the effect of hypothyroidism (hypothyroidism) on learning and memory of young rats (puppies)...

2020-08-13
Revolutionary gene editing technology CRISPR has become the new favorite of scientists

Revolutionary gene editing technology CRISPR has become the new favorite of scientists

When viruses attack bacteria, they cause a defense response against DNA. Biologists use this mechanism to develop genetic engineering techniques....

2020-08-13
How to prepare a frog or toad sciatic nerve-gastrocnemius animal model?

How to prepare a frog or toad sciatic nerve-gastrocnemius animal model?

Experimental principle Frogs or toads and other amphibians have the same basic life activities and physiological functions as warm-blooded animals, and the living conditions of their isolated tissues are easy to master...

2020-08-13