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How to prepare a transgenic animal model of hepatitis C virus (HCV)?

How to prepare a transgenic animal model of hepatitis C virus (HCV)?

The method is mainly to prepare transgenic mice by male pronucleus microinjection....

2020-08-12
How to prepare the animal model of rotation and tremor?

How to prepare the animal model of rotation and tremor?

Modeling materials Animal: rabbit; equipment: rabbit box, JKY-11 electric swivel chair, Rm-6000 eight-channel physiological recorder...

2020-08-12
How to prepare an animal model of depression?

How to prepare an animal model of depression?

1. Modeling materials Animal: healthy purebred mice, male, 4 to 5 weeks old; drug: reserpine.   2. Modeling method Observe for 10 hours after injecting 2mg/kg reserpine into the tail vein of mice....

2020-08-12
How to prepare the selection and application of infectious disease animal models?

How to prepare the selection and application of infectious disease animal models?

The ideal animal model of infectious disease should completely or basically resemble the clinical manifestations, disease process, pathophysiological changes, immunological response and other disease characteristics of human diseases...

2020-08-12
How to prepare animal model of bladder foreign body implantation stone?

How to prepare animal model of bladder foreign body implantation stone?

Anesthetize adult male SD or Wistar rats weighing 250-300 g by intramuscular injection of diazide 5 5 mg/kg and ketamine 50 mg/kg...

2020-08-12
How to prepare an adrenaline animal model?

How to prepare an adrenaline animal model?

According to the strain, intravenous injection of 0.1% epinephrine solution, 0.3 ml/kg body weight of New Zealand rabbits, 5 ml/kg body weight of Wistar rats, and 8-10 ml/kg of Kunming mice...

2020-08-11
【Animal Experiment】-Tree Shrew Hepatitis B Model

【Animal Experiment】-Tree Shrew Hepatitis B Model

Replication method 0.5ml of hepatitis B serum from hepatitis B virus surface antigen (HBsAg) and hepatitis B virus e antigen (HBeAg) was inoculated into trout via femoral vein...

2020-08-11
How to prepare a rat model of experimental pulmonary edema induced by nitrous oxide?

How to prepare a rat model of experimental pulmonary edema induced by nitrous oxide?

Choose a 170-210 g Wistar rat, take 15, 20, 25 μl of N2O410 liquid and pour it into a 120 L poisoning cabinet...

2020-08-11
【Animal Experiment】-Macaque Hepatitis B Model

【Animal Experiment】-Macaque Hepatitis B Model

Replication method Inoculate 2 ml of HBV positive serum intravenously into healthy rhesus monkeys of 2-4 kg, and detect their negative HAV, HBV and HCV markers at the age of 1-3...

2020-08-11
How to prepare an animal model of platelet active factor-induced asthma?

How to prepare an animal model of platelet active factor-induced asthma?

Choose 300-500g of adult male guinea pigs, use normal saline containing 0.25% calf serum albumin to dilute platelet activating factor to 500μg/ml, which may cause guinea pig asthma 1500μg, which may be inhaled at a dose per kg...

2020-08-11
How to prepare an animal model of hepatitis B virus infection?

How to prepare an animal model of hepatitis B virus infection?

Hepatitis B virus (HBV) is a hepatotrophic DNA virus, which is a complex DNA virus. Since the 1970s, we have identified Dane particles (HBV particles) with electron microscopes and discovered HBV particles...

2020-08-11
【Animal experiment】-Rhesus monkey infection model

【Animal experiment】-Rhesus monkey infection model

Breeding method Before the experiment, collect adult experimental rhesus monkeys, weighing 2-5 kg, and screen for HBsAg, anti-HAV and other liver function enzyme activities. After ketamine anesthesia, first collect blood samples and liver tissue biop...

2020-08-11
Genetic engineering animal model of catecholamine action system

Genetic engineering animal model of catecholamine action system

“Ércháfēn\'àn” bāokuò shènshàngxiàn sù, qù jiǎ shènshàngxiàn sù, duōbā\'àn jí qí dàixiè chǎnwù 29/5000 \"Catecholamines\" include epinephrine, norepinephrine, dopamine and their metabolites...

2020-08-11
How to prepare an orphan or separate animal model of depression?

How to prepare an orphan or separate animal model of depression?

Some primates or non-primates have characteristic manifestations such as restlessness, sleep disturbance and crying after separation of offspring...

2020-08-11
How to prepare an animal model of drug-induced depression?

How to prepare an animal model of drug-induced depression?

Drug antagonism: Reserpine can antagonize this effect. This is the earliest study to establish antidepressants, because it was found that monoamine oxidase inhibitors and tricyclic antidepressants can counteract abnormal animal behaviors caused by re...

2020-08-11
How to prepare a transgenic animal depression model?

How to prepare a transgenic animal depression model?

WKY Rats WKY (WistarKyoto) rats are sensitive to various stimuli and show behavior defects in some common behavior tests...

2020-08-11