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What are the steps to extract plasmid DNA by alkaline lysis?

What are the steps to extract plasmid DNA by alkaline lysis?

What are the steps to extract plasmid DNA by alkaline lysis?...

2020-10-14
How to configure the nutrient solution for experiment?

How to configure the nutrient solution for experiment?

How to configure the nutrient solution for experiment?...

2020-10-14
How to do ether anesthesia experiment in rats and mice?

How to do ether anesthesia experiment in rats and mice?

How to do ether anesthesia experiment in rats and mice?...

2020-10-14
What is the method of genotyping?

What is the method of genotyping?

Genotype (genetic type) refers to the type of genes related to the traits contained in the DNA of an organism...

2020-10-14
What is embryonic stem cell method?

What is embryonic stem cell method?

The Martin Evans Institute has successfully cultivated a pluripotent stem cell line from the internal cell mass of early embryos, which can grow in vitro and can differentiate into other cells even if it is reinjected into germ cysts , These cells (i...

2020-10-14
What is the retroviral vector infection method?

What is the retroviral vector infection method?

Studies have shown that retroviruses, especially lentiviral vectors, can effectively introduce foreign DNA into egg cells...

2020-10-14
What is DNA microinjection?

What is DNA microinjection?

DNA microinjection is the earliest, most mature and most widely used transgenic technology...

2020-10-14
What is a point-specific recombination system?

What is a point-specific recombination system?

\"Point-specific recombination system\" includes Cre-LoxP system and FLP-FRT system. So far, the most widely used method is Cre/LoxP-mediated point-specific recombination system...

2020-10-14
What is an inducible gene expression system?

What is an inducible gene expression system?

Inducible Gene Expression System (Inducible Gene Expression System) allows introduced genes to regulate gene expression levels at a specific time or on specific tissues or cell types...

2020-10-14
How to prepare an animal model of transplanted tumor?

How to prepare an animal model of transplanted tumor?

The blood samples of clinical leukemia patients cultured and passaged in the abdominal cavity of mice, bone marrow samples or non-solid tumor ascites samples are equal amounts of balanced salt solution (PBS, etc.)...

2020-10-14
How to extract DNA correctly?

How to extract DNA correctly?

How to extract DNA correctly?...

2020-10-14
How to properly do the tail vein injection operation in rats?

How to properly do the tail vein injection operation in rats?

Tail vein injection in rats is a common operation, but it is a bit difficult, but if there is no guidance, it may be a little confusing at first...

2020-10-14
How to make a genetically modified mouse model?

How to make a genetically modified mouse model?

The selection of donor mice, excessive ovulation and reproduction should be based on the purpose of constructing transgenic animals to determine the type and pedigree of donor mice...

2020-10-14
What is embryonic stem cell technology for laboratory animals?

What is embryonic stem cell technology for laboratory animals?

Stem cell research is one of the fastest growing and most valuable cutting-edge technologies in the field of life sciences in recent years. It was published in Science and Technology in 1999, 2000 and 2003 by the journal Science...

2020-10-14
What is somatic cell nuclear transfer in pigs?

What is somatic cell nuclear transfer in pigs?

CSU-23 culture medium is a phosphate buffer without NaHCO 3. The physiological pH phosphate buffer is composed of dihydrogen phosphate anions and dihydrogen phosphate anions with a molar ratio of 3:1...

2020-10-14
What are the principles of CRISPR/Cas9 technology against AIDS?

What are the principles of CRISPR/Cas9 technology against AIDS?

HIV (HIV) is an effective and cunning retrovirus. When HIV introduces its DNA into the genome of the host cell, it can incubate for a longer period of time and can remain dormant for several years...

2020-10-14