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How to prepare an animal model of otitis media?

How to prepare an animal model of otitis media?

The replication method uses 300-420 g guinea pigs and reflects players normally. First, perform an otoscope examination to check for external and middle ear diseases. Exclude and test separately...

2020-08-08
How to prepare an animal model of orbital fracture with invaginated eyeball?

How to prepare an animal model of orbital fracture with invaginated eyeball?

Breeding method Anesthetize adult cats weighing 2.0 to 3.0 kg by intramuscular injection of 20 mg/kg body weight of ketamine and 2.5 mg/kg disi epa...

2020-08-08
Shìwǎngmó xīnshēng xiěguǎn dòngwù móxíng zěnme zhìbèi? 16/5000 How to prepare an animal model of retinal neovascularization?

Shìwǎngmó xīnshēng xiěguǎn dòngwù móxíng zěnme zhìbèi? 16/5000 How to prepare an animal model of retinal neovascularization?

C57BL/6N mice born 7 days after birth were placed in a 75% oxygen environment, kept at room temperature (23±2) ℃, and returned to the air environment after 5 days...

2020-08-08
How to prepare an animal model of retinal vein occlusion?

How to prepare an animal model of retinal vein occlusion?

Copy method Anesthetize 8-10 kg piglets by intramuscular injection of 0.1 ml/kg of compound ketamine, use compound troponin to dilate pupils and fix the head on the head...

2020-08-08
【Animal model】-animal model of traction retinal detachment

【Animal model】-animal model of traction retinal detachment

Replication method (1) Culture of rabbit skin fibroblasts: Cut a New Zealand rabbit weighing about 2 kg into 1 piece, disinfect the skin with 75% ethanol, and use cornea quhuaning to cut into the dermis to a diameter of 8 mm incision...

2020-08-08
[Dòngwù móxíng]-kǒng yuán xìng shìwǎngmó tuōlí dòngwù móxíng 19/5000 【Animal model】-animal model of rhegmatogenous retinal detachment

[Dòngwù móxíng]-kǒng yuán xìng shìwǎngmó tuōlí dòngwù móxíng 19/5000 【Animal model】-animal model of rhegmatogenous retinal detachment

Reproduction method: No abnormality in eye examination. Fixed New Zealand rabbits weighing 2.0-2.5 kg, flushed with 1% atropine eye drops to dilate the pupils, and 30 mg per mg was injected into the tail vein of the ear to anesthetize the kilogram of...

2020-08-08
[Animal model]-Optic nerve stretch injury model

[Animal model]-Optic nerve stretch injury model

Reproduction method Inject pentobarbital sodium with a body weight of 30 mg/kg body weight into a guinea pig weighing about 700 g, incise the outer edge under an operating microscope, cut the corneal limbus into 360°, cut the conjunctiva and eyeball...

2020-08-08
【Animal model】-Chemical substance induced cholecystitis model

【Animal model】-Chemical substance induced cholecystitis model

Reproduction method Male and female guinea pigs weigh 300-400 grams, weigh 60-kg/kg body weight, once a day, inject lincomycin hydrochloride or 300 mg/kg body weight lithocholic acid intraperitoneally for 7 consecutive days (every day for 15 consecut...

2020-08-07
How to prepare an animal model of liver failure induced by multiple factors?

How to prepare an animal model of liver failure induced by multiple factors?

Replication method Adult male mice or rats 12 hours after fasting, intraperitoneal injection of D-galactosamine (D-Gal) and endotoxin lipopolysaccharide (LPS) 0.1 at a dose of 800-1000 mg/kg body weight mg/kg Body weight: or adult rats, 30 mg/kg body...

2020-08-07
[Animal Experiment]-Phytolithic cholecystitis model

[Animal Experiment]-Phytolithic cholecystitis model

Reproduction method Inject 3% sodium pentobarbital intravenously to male or female rabbits weighing 2-3kg at a dose of 30mg/kg body weight and use sterile drape anesthesia under the xiphoid...

2020-08-07
【Animal model】-Common bile duct ligation plus bacterial infection model

【Animal model】-Common bile duct ligation plus bacterial infection model

Reproduction method Disinfect male or female rabbits weighing 2-3 kg, spread them with a sterile towel, make 3-4 abdominal incisions under the xiphoid process, expose and separate the common bile duct, and remove the common bile duct...

2020-08-07
【Animal model】-Carbon tetrachloride induced liver failure model

【Animal model】-Carbon tetrachloride induced liver failure model

Reproduction method Male and female rats are forced to take orally 10% 40% CCl4 oil solution/kg body weight, or adult dogs are injected intraperitoneally with 50% CCl4 oil solution 0.9 ml/kg body weight, the hair color of the animals after administra...

2020-08-07
How to prepare a liver failure model induced by galactosamine?

How to prepare a liver failure model induced by galactosamine?

Replication method After fasting for 12 hours, adult rats were injected intraperitoneally with D-galactosamine (D-Gal) at a dose of 1.2-1.8 g/kg body weight...

2020-08-07
How do animal models of atherosclerosis and what are their characteristics?

How do animal models of atherosclerosis and what are their characteristics?

In 1908, Ignatowski and colleagues realized that a high-cholesterol diet may be related to the formation of experimental atherosclerosis...

2020-08-07
How to prepare an animal model of liver failure induced by acute liver ischemia?

How to prepare an animal model of liver failure induced by acute liver ischemia?

The adult animal is fixed under anesthesia, the abdomen is opened along the midline of the abdomen, and the inferior vena cava above the renal vein is sutured with purse-string suture with 5-0 vascular suture...

2020-08-07
How to prepare animal models of liver failure induced by liver resection?

How to prepare animal models of liver failure induced by liver resection?

Model method: Adult rats were anesthetized by intraperitoneal injection of ether or ketamine at 100 mg/kg body weight, fixed on the back, and cut the abdomen along a transabdominal incision...

2020-08-07