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【Animal modeling】-Isolation and culture of mouse spleen mesenchymal stem cells by tissue block method

【Animal modeling】-Isolation and culture of mouse spleen mesenchymal stem cells by tissue block method

To establish an efficient and reliable strategy for the isolation and expansion of mouse spleen mesenchymal stem cells by tissue block culture method...

2022-03-15
【Animal modeling】-Observation of lifespan of presenilin-1/presenilin-2 double knockout mice

【Animal modeling】-Observation of lifespan of presenilin-1/presenilin-2 double knockout mice

To observe the survival curve of Presenilin-1/Presenilin-2 double knockout mice (dKO mice), and to provide a reference for further reproduction and the use of this model for the study of Alzheimer\'s disease (AD)...

2022-03-15
【Animal modeling】-Depolarization rebound and regulatory mechanism of glial neurons in the dorsal horn of rat spinal cord

【Animal modeling】-Depolarization rebound and regulatory mechanism of glial neurons in the dorsal horn of rat spinal cord

To study the depolarization rebound and its regulatory mechanism of the colloidal (SG) neurons in the dorsal horn of the spinal cord in rats, in order to provide a reference for the clinical treatment of depolarization rebound related diseases...

2022-03-15
【Animal modeling】-Analysis of clinical characteristics of mice infected with parvovirus

【Animal modeling】-Analysis of clinical characteristics of mice infected with parvovirus

To analyze the distribution law, detoxification and antibody changes of mouse parvovirus in artificially infected mice, and the situation of MVM infection in mice under natural conditions...

2022-03-15
【Animal modeling】-Prokaryotic expression of monkey D-type retrovirus p27 gene and establishment of flow immunofluorescence microsphere detection technology

【Animal modeling】-Prokaryotic expression of monkey D-type retrovirus p27 gene and establishment of flow immunofluorescence microsphere detection technology

To construct the p27 protein of simian D-type retrovirus, and establish a flow immunofluorescence microsphere detection technology for the detection of simian D-type retrovirus...

2022-03-14
【Animal Modeling】-Fluorescence two-dimensional electrophoresis to detect the differences in liver tissue proteomics between male and female mice

【Animal Modeling】-Fluorescence two-dimensional electrophoresis to detect the differences in liver tissue proteomics between male and female mice

To detect the differences in the expression of proteomes in the liver tissue of male and female mice, and to explore the mechanism of gender differences in the occurrence of liver diseases...

2022-03-14
【Animal modeling】-Isolation and identification of mouse CD4+ T cell exosomes

【Animal modeling】-Isolation and identification of mouse CD4+ T cell exosomes

To investigate whether CD4+ T cells have the ability to secrete exosomes, and the possibility of CD4+ T cell exosomes participating in inflammation and tumor immune processes...

2022-03-14
【Animal Modeling】-The mechanism of acute hyperglycemia affecting the first phase insulin secretion in mice

【Animal Modeling】-The mechanism of acute hyperglycemia affecting the first phase insulin secretion in mice

To observe the function and morphological changes of acute hyperglycemia affecting the first phase insulin secretion in mice...

2022-03-14
【Animal Modeling】-Establishment of a fluorescent PCR detection method for mouse polyoma virus and its application in the investigation of infection in naked mole rats

【Animal Modeling】-Establishment of a fluorescent PCR detection method for mouse polyoma virus and its application in the investigation of infection in naked mole rats

To establish a quantitative PCR detection method for polyoma virus, and to investigate the infection of polyoma virus in naked mole rats...

2022-03-14
【Animal Modeling】-Analysis of Transcriptional Levels of CMAH Gene in Different Tissues of Mice

【Animal Modeling】-Analysis of Transcriptional Levels of CMAH Gene in Different Tissues of Mice

The sialic acid and its derivatives contained in mammalian cells mainly include N-acetylneuraminic acid, N-glycolylneuraminic acid and deaminated neuraminic acid. The synthesis of Neu5Gc under the catalysis of acid hydroxylase? In this study, the rel...

2022-03-14
【Animal modeling】-Analysis of genetic characteristics of BABL/c mutant curly hair mice by microsatellite DNA markers

【Animal modeling】-Analysis of genetic characteristics of BABL/c mutant curly hair mice by microsatellite DNA markers

To analyze the genetic differences between BALB/c mutant curly-haired mice and normal BALB/c mice using microsatellite DNA marker technology...

2022-03-14
【Animal modeling】-Establishment and application of Salmonella LAMP detection method in tree shrew feces

【Animal modeling】-Establishment and application of Salmonella LAMP detection method in tree shrew feces

To establish a loop-mediated isothermal amplification detection method for Salmonella in tree shrew feces, and to make a preliminary application of the method...

2022-03-14
【Animal Modeling】-Based on the 3.0 T magnetic resonance imaging system to preliminarily observe the morphological and anatomical structure of the WHBE rabbit brain

【Animal Modeling】-Based on the 3.0 T magnetic resonance imaging system to preliminarily observe the morphological and anatomical structure of the WHBE rabbit brain

Based on 3.0 T magnetic resonance imaging (MRI) technology, the morphological and anatomical structure of the WHBE rabbit brain was observed in vivo, and the basic biological data of the WHBE rabbit brain imaging were accumulated, so as to provide ba...

2022-03-12
【Animal Modeling】-Establishment of a Fluorescent Quantitative Detection Method for Suspected Taylor\'s Virus in Rats

【Animal Modeling】-Establishment of a Fluorescent Quantitative Detection Method for Suspected Taylor's Virus in Rats

To establish a rapid and accurate method for the clinical detection of suspected Theiler\'s-like virus of rats (TLV) in rats, using TaqMan probe fluorescence quantitative polymerase chain reaction (qPCR) technology, specific Sexual detection of TLV v...

2022-03-12
【Animal modeling】-Microsatellite DNA analysis of the genetic status of 24 domestic inbred strains of mice

【Animal modeling】-Microsatellite DNA analysis of the genetic status of 24 domestic inbred strains of mice

To analyze the genetic status of 24 domestic inbred mice using microsatellite loci...

2022-03-12
【Animal modeling】-Establishment and application of multiplex PCR method for four pathogens in experimental animals

【Animal modeling】-Establishment and application of multiplex PCR method for four pathogens in experimental animals

To establish a multiplex PCR method for the rapid detection of four experimental animal pathogens, Pasteurella multocida, Bordetella bronchiscus, Mycoplasma and Klebsiella pneumoniae...

2022-03-12