【Animal Modeling】-Chemical Stimulation Method-induced Pain Model

Modeling material animal: rabbit; drug: saturated potassium chloride solution; device: DC potassium ion pain meter...

2021-11-15

【Animal Modeling】-Ovariectomized Osteoporotic Tree Shrew Model

Reproduction of a postmenopausal osteoporotic tree shrew model with surgical bilateral ovarian castration...

2021-11-15

【Animal Modeling】-The effect of whole body X-ray irradiation on the hematopoietic immune system function of type 2 diabetic KKAy mice

How to observe the destructive effect of whole body X-ray radiation on the hematopoietic immune system function of type 2 diabetes model KKAy mice and compare it with control C57 mice?...

2021-11-15

【Animal Modeling】-The role of MicroRNA-137 and AngⅡ in cardiac remodeling in spontaneously hypertensive rats

To explore the role of microribonucleic acid 195 (MicroRNA-137, miRNA-137), TGF-β1/Smads signaling pathway and angiotensin II (AngII) in spontaneously hypertensive rats (SHR) cardiac remodeling...

2021-11-15

[Animal Modeling]-Establishment of post-traumatic epilepsy animal model by microinjection

Modeling material animal: Wistar rat, male, weight 200-250g, medicine: isopentobarbital, FeCl3 solution, equipment: rat stereotactic system, wireless Bluetooth EEG monitoring system, high-speed dental drill, dental tray powder, micro syringe...

2021-11-15

Long-term effects of sublethal γ-ray irradiation on the hematopoietic immune function of mice

Observe the changes of hematopoietic and immune function indexes of C57BL/6J mice 6 months after 4Gy and 6Gy whole body irradiation...

2021-11-15

【Animal Modeling】-Animal Model of Retinal Neovascularization

Replication method (1) Place 7-day-old C57BL/6N mice in a 75% oxygen environment, control at room temperature (23±2) ℃, and return to the atmosphere after 5 days...

2021-11-15

【Animal Modeling】-Animal Model of Traumatic Articular Cartilage Damage Repair

　　Reproduction method Anesthetize 4-5 months old New Zealand rabbits with intraperitoneal ketamine of 50 mg/kg body weight, and then fix them on the surgical plate...

2021-11-15

【Animal Modeling】-Acute Ulcerative Colitis Induced by Acetic Acid Animal Model

Reproduction method Anesthetize adult male rats by intraperitoneal injection of sodium pentobarbital, fasting for 16 hours and depriving water, slowly insert the polyethylene catheter from the colon at 8 cm from the anus into 1.5 to 2 ml of 8% acetic...

2021-11-15

【Animal Modeling】-Preliminary Study on the Tolerance of Naked Mole Heart to 60Co γ-ray Radiation

Observe and analyze the changes in the morphology, structure and tissue biochemical indexes of Hadakadebanezumi\\\'s heart by 60Coγ-rays, and preliminary study the tolerance of Hadakadebanezumi\\\'s heart to 60Coγ-rays...

2021-11-15

【Animal Modeling】-How to build an animal model of inhalation of atmospheric fine particles?

Establish an animal model of physical injury caused by long-term inhalation of atmospheric particulate matter (PM2.5) aerosols...

2021-11-15

【Animal Modeling】-A 549 green fluorescent nude mouse model of lung adenocarcinoma

Establish a lung adenocarcinoma A549 model inoculated subcutaneously in green fluorescent nude mice to study the source of vascular endothelial cells in the process of lung adenocarcinoma tumor angiogenesis...

2021-11-12

【Animal Modeling】-Isolation and culture of primary tree shrew corneal stromal cells

To explore a simple and easy method to isolate and culture tree shrew corneal stromal cells in vitro quickly...

2021-11-12

【Animal Modeling】-Directed breeding and identification of Mfge8 knockout homozygous mice and its correlation with autoimmune diseases

To breed and identify Mfge8 knockout C57BL/6 mice, obtain homozygous (Mfge8-/-) mice, and compare the serological and histological changes of Mfge8-/- mice...

2021-11-12

【Animal Modeling】-Based on the Tet-On system to construct a lentiviral vector for the Alb promoter to regulate the expression of porcine uPA transgene

Using Tet-On gene expression regulation system to construct a lentiviral vector pLVX-Alb-TetOne-TRE-puPA-T2A-CopGFP(pLATTPUTG )...

2021-11-12

【Animal Modeling】-CRISPR/Cas9 system mediates reproduction and identification of Slc6a6 knockout rats

Use CRISPR/Cas9 technology to construct taurine transporter gene (solute carrier family 6 member 6, Slc6a6) knockout rats, breed and identify them, and provide a stable rat model for studying the effects of taurine deficiency on neurological diseases...

2021-11-12