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Prepare sustained-release endotoxin polymer pellets by the replication method: wash the ethylene-vinyl acetate polymer beads with high-purity hexanal to spectrophotometric purity, and dissolve the polymer in methyl chloride at room temperature to 10%...
2021-03-29Reproduction method When using copper chloramine hydrochloride (50 mg/kg body weight) and chlorpromazine (10 mg/kg body weight), New Zealand rabbits weighing 2.5 to 3.5 kg and weighing more than 4.5 kg are unlikely to induce corneal neovascularizatio...
2021-03-29Breeding method 30 mg/kg body weight of pentobarbital sodium was injected intraperitoneally into male Wistar rats weighing 200 to 220 g, and fixed on the operating table in supine position...
2021-03-26Breeding method Inject cyclophosphamide solution into a New Zealand female rabbit weighing about 1.5 kg at a dose of 8.0×10-2 g/kg from a guinea pig intravenously. After 6 hours, use 0.1, (Xinjieerjie) to treat the rabbit near the vulva of the rabbit...
2021-03-26Radical nephrectomy (1) Replication method An adult rat weighing about 250 g is anesthetized by intraperitoneal injection of 30 mg/kg body weight of pentobarbital sodium...
2021-03-26Replication methods ① Kanamycin model: The rats were deprived of water for 24 hours before the experiment, and then intramuscularly injected with kanamycin at a dose of 200 mg/kg body weight. After 24 hours, blood was collected to measure BUN and SCr...
2021-03-26After renal ischemia/reperfusion, the content of SOD in the kidney tissue of model rats decreased, and the content of MDA increased significantly, and the content of both showed more obvious gradual changes as the reperfusion time continued....
2021-03-26Reproductive methods Gentamicin (GM) was injected subcutaneously into adult rats weighing 200 to 260 g twice a day for 2 consecutive days at a weight of 400 mg/kg. Rat urine was collected on days 2, 5, 8, 11, 15, 15, 22, and 29 after the first inject...
2021-03-26Breeding method The anesthesia method of sodium pentobarbital is to inject 30 mg/kg of body weight intravenously into New Zealand rabbits weighing 2 to 3 kg, fix them on the back operating table, and regularly disinfect the abdominal skin...
2021-03-26Adult rats weighing 250～300g are anesthetized by intraperitoneal injection of 5% chloral hydrate. After anesthesia, they are placed in the supine position and fixed on the operating board....
2021-03-26Rats weighing about 200g, prohibit drinking water for 24 hours, and tie the penis of the rat with a thin thread in advance. Under ether inhalation anesthesia, the animal was placed on the back and fixed on the surgical board. The abdominal skin was r...
2021-03-26Replication method For adult male mice weighing 25 to 30 g, HgCl2 was prepared into 0.125 g/L Hg2 + solution and physiological saline, and then HgCl2 ml/kg body weight was injected from the tail vein of the mouse at a dose of 10 (the dose was about L...
2021-03-26The replication method mainly uses transgene or gene knockout technology to study the role of changes in specific gene expression products in the development of asthma...
2021-03-25After 5 to 7 days of adaptive reproduction of adult guinea pigs, 10% ovalbumin saline was injected intraperitoneally at a weight of 200 mg/kg and inhaled intraperitoneally on the 15th day...
2021-03-25In the modeling experiment, mice are placed in a 10 L lower mouth bottle, a 1.5 cm diameter vent hole is left on the cap, the lower mouth is connected to the tee, and the other end is connected...
2021-03-25During the experiment, the experimental rats (male and female) were placed in a fume hood, and the cabinet was exposed to smoke (twice a day, 16 cigarettes/hour per day) and smoke concentration, and controlled...
2021-03-25